Protein purification

The protein purification service continues from an existing gene synthesis or protein expression order to submit purification and QC requests. The current online page has no direct entry without a source order—a qualifying upstream order is required first.

Open protein purification

1. After signing in, open wet-lab services from the left of the agent workbench.
2. Find Protein purification.
3. Check whether the Select source order to continue button is available.

When the current account has no qualifying order, the button is disabled and the page prompts:

Complete gene synthesis or protein expression first

This means you cannot bypass the upstream flow by typing an arbitrary order number, nor create a purification order from a blank form.

Which orders qualify as sources

The online entry currently summarizes the requirement as a completed gene synthesis or protein expression order. Whether continuing is actually possible also depends on the order's current status, target protein, and deliverable material.

Before starting, confirm:

• The source order belongs to the current account or organization.
• It has reached a status the page allows to continue into purification.
• Its gene, protein, tags, and material really are this purification's target.
• The upstream delivery form can enter the purification workflow.
• The source material quantity and quality support the planned purification and QC.

To complete upstream services first, see gene synthesis or protein expression.

Find and select the source order

Once a source order qualifies:

1. Select Select source order to continue on the wet-lab services home page.
2. Find the target order in the source order list.
3. Verify order number, service type, target protein, and status.
4. Select the correct order to enter the purification form.

Do not choose the source by creation time alone. Similarly named orders may differ in sequence, tags, or expression batch.

Before you start

Information	What to prepare
Source order	Order number, service type, status, and upstream deliverables
Target protein	Name, sequence, database ID, and batch
Starting material	Sample type, volume, concentration, total amount, storage
Tag information	Tag type, terminus, and whether removal is needed
Purification goal	Purpose, target purity, concentration, and delivery amount
Quality requirements	Endotoxin, aggregation, activity, or other test conditions
Buffer	Composition, pH, salt, and necessary additives
Downstream use	Structural studies, binding assays, cell experiments, or other

These shape the purification route, tests, sample-loss risk, and quote. When unsure, confirm the plan with the platform before submitting.

Confirm the target protein

After selecting a source order, first verify the target protein information the page brings in.

Focus on:

• Target protein name and sequence version.
• Species, construct, and source order.
• Expression tag and its terminus.
• Whether there are signal peptides, fusion partners, or cleavage sites.
• Whether the starting sample is supernatant, pellet, lysate, or other.
• Sample amount, concentration, and storage.

Auto-filled source-order information still needs manual review—wrong tags, host, or batch upstream directly corrupt the purification plan.

Configure purification parameters

Fill in the purification requirements based on the target protein and sample.

Make explicit where possible:

• The target purification method, or permission for the platform to recommend one.
• Whether to use the tag for affinity purification.
• Whether the tag must be removed, and the cleavage conditions.
• Whether refolding or other special handling is allowed.
• Target buffer and components that must not be used.
• Cold, light-protected, sterile, or other handling requirements.

Without solid grounds, do not fix a process just to finish the form. State goals and constraints and let the platform confirm the route before quoting.

Set quality and delivery requirements

Acceptance after purification should use measurable criteria.

Requirement	Suggested wording
Purity	Target percentage plus the assay method
Concentration	Value, unit, and allowed range
Delivery amount	Total mass, volume, or the delivery form the page offers
Endotoxin	Threshold, unit, and downstream use
Aggregation	Whether monomer ratio or aggregation testing is needed
Activity	Whether functional testing is needed, and the method
Buffer	Composition, pH, salt, and additives
Aliquoting	Volume per tube, tube count, temperature, freeze-thaw limits

Descriptions like "high purity", "high concentration", or "for experiments" are not acceptance criteria. Add values, units, assay methods, and downstream use.

Enter information confirmation

After completing required fields, select the page's next-step button to enter confirmation.

At confirmation, check:

• The source order and target protein correspond.
• Sample type, amount, and batch are correct.
• Tag handling matches the upstream construct.
• Purity, concentration, delivery amount, and assay methods are complete.
• Endotoxin, aggregation, and activity requirements match the application.
• Buffer, aliquoting, storage, and shipping requirements are clear.

Return and fix problems before entering the quote.

Quote and submission

The purification plan, sample nature, quality standards, and test items all affect the quote and timeline. The confirmed quote and mutually agreed scope after information confirmation govern.

A source order is not a purification order

Selecting a source order only creates the upstream link. A business flow forms only after the purification form, information confirmation, quote confirmation, and final submission. Do not read the upstream order status as purification having started.

When no source order is available

If the button is disabled:

1. Check whether the account has completed gene synthesis or protein expression orders.
2. Check upstream order status in order history.
3. Confirm the order belongs to the current account or organization.
4. Complete gene synthesis or protein expression first, per your R&D stage.
5. If you have external samples or cannot continue from a platform order, use other custom services or contact the platform.

Do not fabricate an upstream order to enable the button.

Troubleshooting the form

Situation	How to check
Select source order to continue unavailable	Check for gene synthesis or expression orders meeting the status requirement
Source order list empty	Check account, organization, order type, and order status
Target protein information inconsistent	Return to the source order; verify sequence, tags, construct, and batch
Unsure of the purification method	State goals and constraints; ask the platform to confirm before quoting
Cannot fill in quality requirements	Add values, units, assay methods, and downstream use
Standard form cannot take external samples	Use other custom services or confirm with the platform
Cannot enter confirmation	Check all required fields, units, and the source-order link

For status and progress after a purification order is created, see Order workflow and status.